The investigation of toxicity caused by the degradation process on occluding of dentin tubules with pure and silver-doped nano- hydroxyapatite
Umit ERDEM Mustafa B. TURKOZ Serdar BAĞLAR Mustafa DOGAN Saffet NEZIR
AbstractAbstract In recent years to occlude the dentinal tubule orifices has become the most discussed treatment of the dentine hypersensitivity [1]. For a while, there is a groving interest in developing materials with a bioactive potential that could cover dentinal tubules and reduce the fluid flow within the tubules [2]. Hydroxyapatite (HAp) is one of the main structures of dental hard tissues, exhibits excellent bioactive properties as inducing the natural response of cells and the mineralization process [3]. Although HAp antibacterial resistance is very limited [4-5]. The synthesis of Ag+ doped hydroxyapatite with antibacterial properties is great interest in new biological studies [6]. In this study, both pure HAp and Ag+ doped HAp were produced by precipitation method [6]. It is aimed to increase the antimicrobial effects with Ag+ doped production. At the end of the production FTIR, SEM and degradation studies were made to evaluate the physical characterization. In addition, cytotoxicity studies were conducted to evaluate toxicological properties. The FTIR and SEM studies were also conducted before and after the degradation to assess the physical activity of the treated surface. All samples evaluated in our study were found to be an effective occluding on dentin tubule. According to degredation results, the occluding material was found to have a strong chemical bond structure. Both pure HAp and Ag+ doped HAp materials, has a positive contribution on viability, does not cause toxicity. Furthermore Ag+ doped hydroxyapatite treatment is a positive effect on antibacterial properties. Antibacterial activity of the materials related to Escherichia coli, Staphylococcus aureus, Candida albicans was taken high resistant observed in Ag+ doped hydroxyapatite sample comperad pure hydroxyapatite.